Biomolecule stability is a key concern in investigating its properties and how it might be developed towards industrial application. It is surprising the number of contexts where this simple comparative measurement can offer deep insight into development direction. Below are some examples of where QUBES has been applied, but this is not an exhaustive list. Contact us about any of our examples or if you would like to understand how QUBES could address your research and development needs.
Antibody stability prediction
Therapeutic antibodies offer some of the greatest opportunities for healthcare and some of the greatest challenges in getting to patients. QUBES analysis can determine which antibody derivative is most stable following forced degradation studies. But, more importantly, we have discovered through a pharma collaboration that QUBES analysis can predict which antibody derivative will be the most stable under the proposed forced degradation studies.
Biomolecule excipient choice
Excipient choice for biomolecule stabilisation is a combination of rational choice, with a heavy leaning on empirical evaluation. QUBES analysis offers a faster assessment of stability changes and therefore the potential for a researcher to explore more conditions. The technique is also tolerant of all non-protein excipients, even at formulation concentrations of excipient and biomolecule.
Quality control is often something that only happens on a batch-to-batch basis, but there is merit in a researcher checking more frequently. We have worked with partners to show that structure and function assay data correlates with QUBES analysis data showing batch-to-batch equivalence. QUBES offers a researcher a simple test that can be deployed more frequently to ensure that the biomolecule of interest is as expected prior to study.