Red Edge Excitation Spectroscopy

Red edge excitation spectroscopy (REES) is a spectroscopic technique where emission spectra are captured for a given excitation wavelength. Changes in these fluorophore emission spectra relate to the dynamics of the fluorophore's chemical environment. For biomolecules, the technique relies on just one of the endogenous fluorophores, tryptophan. Experimentally, REES data capture is performed using a standard fluorimeter that allows for fine control over excitation and emission wavelengths. It is these resulting spectra that are analysed by the QUBES software tool to result in a comparative analysis of protein stability.

QUBES analysis

Biomolecule stability is a critical parameter in determining its developability. QUBES is a comparative analytical technique allowing the stability of biomolecules and their derivatives. The technique is based on a novel, benchmarked, photophysical model of red edge excitation spectroscopy data, developed by Dr Chris Pudney at the University of Bath. This rapid insight into biomolecule stability allows researchers to evaluate potential biomolecule development paths more swiftly and confidently.